Education
2009, B.S University of Iowa, Biochemistry
2015, Ph.D. Harvard University, Microbiology and Immunobiology
I study the disease-causing bacteria Clostridioides difficile or C. diff for short. C. diff causes a severe gastrointestinal illness in individuals with a compromised microbiome or in the elderly and is the leading cause of hospital acquired infections in the US. C. diff is an obligate anaerobe, meaning it cannot survive in oxygen. In order to transmit infection between individuals, C. diff relies on a metabolically dormant cell type called an endospore. Endospores have a dehydrated cytosol where the DNA is tightly packaged and multiple protective layers that make them resistant to a lot of environmental stressors. For C. diff to cause disease, endospores need to be ingested, survive passage through the stomach, sense when they have reached the small intestine, and exit their dormant state through a process called germination.
I’m interested in how C. diff endospores sense they have reached the small intestine and trigger the process of germination. We use a combination of genetic and biochemical techniques to look at the role of specific proteins involved in sensing germination signals and triggering the exit from the dormant state.
There are several ways to get involved in research in the lab and they all start with stopping by my office and/or sending me an email! You do not need to have a project in mind to come work in the lab! I will have ongoing projects that you can contribute to. I love training students in the lab and teaching them what research is all about!
How you get involved in the lab depends on your availability, experience level, and what you hope to get out of your experience. Options include:
- Volunteering
- This option is the best for students getting started in research in their second and/or for students who just want to learn a little bit about what microbiology research looks like!
- Work out a schedule with Dr. Rohlfing to come into the lab to learn the techniques we use in the lab!
- This is the most flexible option with the lowest level of commitment. Time in the lab will be ~1-2 hours/week
- Directed research
- Get course credit for doing research! Students typically do directed research in their senior year.
- Students doing directed research will work with Dr. Rohlfing to pick a project related to C. diff endospore formation or germination that interests them
- There are two directed research options. BI421/BI422: 4 credits spread out over two semesters OR BI423: 4 credits in one semester
- INBRE student researcher
- Get paid to do research during the school year!
- Up to 10 hrs/week
- Students doing INBRE research will work with Dr. Rohlfing to pick a project related to C. diff endospore formation or germination that interests them
- This is a competitive program that requires an application during the fall semester
- INBRE summer research program
- Get paid to work in the lab over the summer for up to 40 hrs/week!
- This is a competitive program that requires an application during the spring semester. If you are interested in summer research in the lab, please contact Dr. Rohlfing in the first half of the spring semester to discuss potential projects.
- Honors program summer research
- An option for students in the Honors Program to work in the lab over the summer and receive a stipend.
- This is a competitive program that requires an application during the spring semester. If you are interested in summer research in the lab, please contact Dr. Rohlfing in the first half of the spring semester to discuss potential projects.
- Honors program thesis research
- This is a flexible option for Honors Program students who are interested in writing their senior thesis on C. diff or a related topic
- Research can be completed in the lab or can be based on a literature review
BI103 General Biology
BI336 Biochemistry
BI211 Genetics for Healthcare
BI318 Microbiology for Majors